The Zhang Wenhao research group of the Institute of Botany of the Chinese Academy of Sciences cooperated with domestic and foreign units to overcome the difficulty of high heterozygosity of the alfalfa genome by integrating the third-generation, second-generation and Hi-C sequencing data, and spliced the high-quality alfalfa genome (904.13 Mb, scaffold N50 99.39 Mb), the researchers revealed the stress resistance mechanism of alfalfa through further analysis of gene family expansion, transcription factors, gene retention/loss during domestication, stress-related SNPs and stress response genes.
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