Recently, the rice functional genomics innovation team of China Rice Research Institute revealed that a glycine rich protein lsl1 is involved in the regulation of chloroplast redox homeostasis mechanism, thereby affecting the yield and quality of rice. Relevant research results were published in Science China Life Sciences.
Chloroplast is an important place for plant photosynthesis, and its energy transfer and the maintenance of redox homeostasis are very important for plant development. Abnormal chloroplast development usually leads to abnormal grain development in rice, resulting in yield reduction and quality damage.
In this study, a plaque like mutant lsl1 was isolated by EMS mutation, which showed a plaque like phenotype in the whole growth period. Physiological experiments showed that reactive oxygen species accumulated excessively in lsl1, and the activity of key enzymes to maintain ROS homeostasis was seriously maladjusted. TUNEL and comet experiments showed that abnormal DNA degradation and cell death occurred in the lsl1 mutant. The chloroplast development of lsl1 mutant is abnormal. Combined with transcriptome data, lsl1 is involved in photosynthesis and chlorophyll synthesis. Abnormal chloroplast results in impaired photosynthesis and decreased grain size and quality of rice.
To further explore the potential molecular mechanism of lsl1, the interaction screening of lsl1 protein was carried out through yeast double hybrid experiment, and two chloroplast development related proteins pap10 and PSAD were identified. Luc and BiFC experiments also confirmed this interaction. It is worth noting that the mutant lsl1 protein lost the ability to interact with PSAD protein. It can be concluded that lsl1 interacts with pap10 and PSAD to maintain the normal development of chloroplasts and cells, thus determining the yield and quality of rice.
The research was supported by National Natural Science Foundation of China and other programs.