In recent years, with the holding of rape flower festivals around the country, the ornamental value of rape flowers has been paid more and more attention by breeders. Domestic breeding units have successively bred new varieties with pink, rose red, apricot red, crimson and other flower colors, but the formation mechanism of these red flower colors is not clear. After successfully analyzing the molecular mechanism of flower color formation of white (New Phytologist, 206:1513-1526) and orange (the plant journal, 104:932-949), recently, the rape genetics and breeding team of Huazhong Agricultural University published an online report entitled”genetic and multi omics analysis reveal bnaa07.pap2in-184-317 as the key gene CO” in the Journal of experimental botanynferring Anthocyanin-baSed color in Brassica napus flowers”reveals the molecular mechanism of flower color formation of rape red line.
In this study, the pink flower material was created from the apricot safflower line selected by Zhejiang Academy of Agricultural Sciences, and the explicit and implicit relationship between the flower colors of various Brassica napus, including white, yellow, apricot red and pink, was clarified. The metabolome analysis of petals of different flower colors showed that apricot red flower colors were formed by the accumulation of yellow lutein and red anthocyanins, while pink flower colors were formed by the accumulation of colorless carotene and red anthocyanins. The content of cyanidin-3-o-glucoside in anthocyanins is the key to the accumulation of red pigment in petals. Transcriptome analysis showed that the structural genes of anthocyanin synthesis pathway and three key transcription factor genes bnamybl2, bnaa07.pap2 and bnatt8 were significantly up-regulated in apricot and pink flowers compared with yellow or white flowers. Based on the results of BSA SEQ and RNA SEQ, the key gene bnaa07.pap2 for the formation of red flower color was cloned. Bnaa07.pap2 is almost not expressed in yellow or white flowers, but highly expressed in apricot or pink flowers. The transgenic results showed that the insertion of two fragments in the promoter region of bnaa07.pap2 was the reason for the activation of the gene expression, and the expression of bnaa07.pap2 activated the expression of the entire anthocyanin pathway gene, promoted the accumulation of red anthocyanins in the petals, and finally led to the formation of red flower color in Brassica napus. In addition, through the hybridization and breeding of Brassica napus with different flower colors, the team created red color materials with different depths.
This research result was also approved by Diarmuid S.? ‘ The special comment”CIS regular variation expands the colour palette of the Brassicaceae” written by Professor maoil é idigh for this article further interprets the significance of this study and highly evaluates its research results.
Ye Shenhua, a doctoral candidate from the school of plant science and technology of Huazhong Agricultural University, was the first author of the paper, Wenjing, an associate professor of rape genetics and breeding team, was the corresponding author, and Hua Shuijin, a post scientist of modern industrial technology system and a doctor of Zhejiang Academy of agricultural Sciences, and Fu Tingdong, Shen Jinxiong, Tu Jinxing, Ma Chaozhi, Yi Bin, Zhao Lun and other professors from Huazhong Agricultural University participated in the study.