Recently, the pesticide and molecular target team of the school of horticulture and plant protection of Yangzhou University in the international famous journal InternatioNal Journal of Molecular Sciences published a publication entitled transcriptome Analysis Reveals Crosstalk between the Abscisic Acid and JasmoNIC acid signaling pathways in rice mediated defense against Nilaparvata lugens. This study revealed that abscisic acid (ABA) and jasmonic acid (Ja) jointly regulate rice resistance to brown planthopper through transcriptome analysis of rice damaged by brown planthopper.
Oryza sativa, a gramineous plant, is an important food crop in the world, providing rations for nearly 50%of the global population. According to the current growth of the global population, it is estimated that in 2025, the global output will need to increase by 60%to meet the normal life of human beings. Its output is closely related to people’s daily life and national economy. Because rice is a plant that cannot get rid of local environmental pressure, it is the most urgent problem in the world to solve the impact of biological and abiotic stresses on rice output.
Nilaparvata lugens, belonging to Hemiptera, Homoptera and planthopper family, has a high growth rate and strong environmental adaptability. It is easy to outbreak in large areas and can migrate long distances. Brown Planthopper is one of the most destructive pests in rice growing areas in Asia and Southeast Asia. It can directly feed on the phloem of rice, lay eggs in the stem and spread the virus. At present, it has become the primary pest of rice in China. Therefore, it is of great significance to improve the resistance of rice to pests and select insect resistant varieties.
Plant hormones are of great significance in plants. The specific elicitors in the secretion produced by insects when they lay eggs on plants or eat plants will activate a variety of plant hormone signal pathways in plants. These signal pathways further activate the expression of defense genes and the production of direct and indirect defense compounds, so that plants show insect resistance. Plant hormones play an indispensable role in regulating growth and development and participating in plant stress resistance. They can be induced by external environmental stress, and often regulate various physiological reactions at low levels in plants. However, it is still unclear how plants coordinate their fluctuating growth defense. Abscisic acid (ABA) and jasmonic acid (Ja) are two important plant hormones, which mainly realize the active regulation of defense function and growth and development.
This study aims to clarify that rice achieves the purpose of resistance to brown planthopper by spraying exogenous hormones to affect the expression of hormones in the body. By analyzing the differential genes of ABA and JA hormone pathways, the differential changes of related insect resistant transcription factors and other genes that may affect the performance of plant insect resistance, it is clear whether there is interaction between hormones, so as to regulate related transcription factors and other insect resistant genes to achieve the purpose of insect resistance, It provides a new idea for understanding the molecular mechanism of insect resistance in rice.
There were 2727 differentially expressed genes (DEGs) treated with BPH and BPH + ja; 1674 genes were up-regulated and 1053 genes were down regulated. Two groups (BA and BJ) regulated 1223 genes; 519 genes were up-regulated and 704 genes were down regulated. By analyzing the transcriptome of rice damaged by brown planthopper, a complex network of ABA and JA coordinated regulation of rice resistance to brown planthopper was constructed.
Li Jitong, doctoral candidate of pesticide and molecular target team of our hospital, first author; Professor liujinglan from the pesticide and molecular target team of our hospital is the corresponding author. This research was supported by the National Natural Science Foundation of China.
Our pesticide molecular target and environmental toxicology team has been engaged in basic and applied basic research on pesticide toxicology for a long time. In the past five years, we have mainly carried out research on the relationship between the structure and function of pesticide targets, the function and mechanism of ABA in rice resistance to brown planthopper, the environmental toxicology of new photoactivated pesticides, and the development of green biological agents based on nano carriers.