Recently, the innovative team of agricultural product quality and safety risk assessment at the Institute of agricultural quality standards and testing technology, Chinese Academy of Agricultural Sciences, studied and clarified the molecular mechanism of bisphenol A substitutes, a new endocrine disruptor, by inducing heterogeneous responses in brain and intestinal cells, which leads to neurotoxicity and metabolic abnormalities. Relevant research results were published in Environmental Science and Technology (environmental Science & Technology) and Environment International）》。
Environmental endocrine disruptors such as bisphenol A are a kind of important new pollutants, and their continuous exposure is one of the important inducements leading to a variety of human diseases. After the use of bisphenol A was restricted, bisphenol F and bisphenol AF and other substitutes were found to have equivalent or even stronger toxic effects than bisphenol A, which attracted widespread attention.
Using zebrafish as a model, researchers identified that long-term exposure to bisphenol A substitutes would bring cognitive impairment and liver fatty and other toxic effects to fish. Further using single-cell transcriptome sequencing technology to analyze the toxic mechanism of bisphenol A substitutes, it was found that the brain cell composition of cognitive abnormal zebrafish has changed significantly, including the decline in the proportion of neurons and the increase in the proportion of immune cells such as microglia, which may lead to cognitive impairment. Through the single-cell transcriptome analysis of intestinal tissue, it was found that the proportion of zebrafish intestinal epithelial cells increased, accompanied by the activation of lipid absorption genes. The above changes may cause excessive absorption of lipids in the intestine, and then cause liver fatty.
The research was supported by the National Natural Science Foundation of China and the science and technology innovation project of the Chinese Academy of Agricultural Sciences.