Recently, the tea plant genetics and breeding team of the Tea Research Institute of the Chinese Academy of Agricultural Sciences has made new progress in the study of nitrate transporter genes in tea plants. The relevant research results are”systematic investigation and ex”Pressure profiles of the nitrate transporter 1/peptide transporter family (NPF) in tea plant (Camellia sinensis)”was published in the internationally renowned journal internationAL JOURNAL OF MOLECULAR SCIENCES》。
Tea is a leaf crop, which has a great demand for nitrogen fertilizer, but also has the problem of low utilization rate. NPF (nitrate transporter 1/polypeptide transporter family, nrt1/ptr family) plays an important role in nitrate transport. In this study, 109 NPF genes were identified from the tea genome, and they were divided into 8 subfamilies according to their sequence characteristics and phylogenetic relationships. Several hormones, cis acting elements of stress response and transcription factor binding sites were identified in the promoter region of tea NPF gene. Collinearity analysis showed that multiple replication types jointly promoted the formation of NPF gene family in tea. Selection pressure analysis showed that purification selection played a leading role in the evolution of NPF gene in tea plants. The distribution of NPF family members showed that eight NPF subfamilies had been formed before the differentiation of dicotyledon and monocotyledon. The expression of NPF genes in tea plants under different concentrations of nitrate nitrogen showed that most NPF genes responded to nitrate nitrogen. Subcellular localization showed that both csnpf2.3 and csnpf6.1 were located on the plasma membrane, which was consistent with the characteristics of transmembrane proteins involved in NO3 transport. This study provides a theoretical basis for further study on the evolution and function of NPF gene in tea plant.
Postdoctoral wangyongxin of our institute is the first author of the paper, and researcher wangliyuan and researcher Cheng Hao are the corresponding authors. The research was supported by the National Natural Science Foundation of China, the special project for the construction of modern agricultural industrial technology system, and the major science and technology project for the breeding of new tea varieties in Zhejiang Province.