Functional characteristics of fungal extracellular membrane protein CFEM revealed by Plant Protection Institute of Chinese Academy of Agricultural Sciences
By: Date: 2022-07-19 Categories: foodtechnology Tags: ,
Recently, the innovation team of grassland pest monitoring and prevention and control of Institute of plant protection, Chinese Academy of Agricultural Sciences published an online publication entitled”bioinformatics analysis and functio” in the Journal of funginal characterization of the CFEM proteins of  meTarhizium anisopliae”. In this paper, the CFEM family genes of entomopathogenic fungus Metarhizium anisopliae were systematically described.
 
The interaction between entomopathogenic fungi and plants can maintain their own population advantage, improve plant nutrient absorption, enhance their disease resistance, stimulate plant endogenous metabolites such as phenolic compounds, or increase the expression of salicylic acid and jasmonic acid pathway defense genes, and improve plant systemic resistance, but its action factors are not clear. The common fungal extracellular membrane protein (CFEM) is widely discussed in fungi. Its structure is similar to the epidermal growth factor (EGF) – like domain, and it acts as an extracellular receptor or signal transducer or adhesion molecule in host pathogen interaction. It has also been reported that CFEM domain proteins may be used as effectors to regulate plant resistance responses. However, in Metarhizium anisopliae, there is no report of related family genes.
 
Using Pfam database and NCBI comparative analysis, 13 CFEM domain proteins were successfully cloned and identified in Metarhizium anisopliae. The functional characteristics of six candidate effector CFEM proteins were preliminarily explored. Subcellular localization showed that five proteins (macfem81, 85, 87, 89, 90) were functionally localized in the plasma membrane, while macfem88 had signals in both the nucleus and the plasma membrane. Macfem81, 85, 88 and 89 were significantly up-regulated at the conidial stage. Immune function verification found that the six effectors could not inhibit the programmed cell death of Benxi tobacco induced by INF1, but macfem85 and macfem88 could induce mild allergic reactions of tobacco. The results are helpful to understand the general situation of CFEM family genes in entomopathogenic fungi, and provide basic materials and research guidance for further exploring the role of CFEM protein in the interaction between Metarhizium anisopliae and plants.
 
The Institute of plant protection of the Chinese Academy of Agricultural Sciences is the first unit to complete the paper, with doctoral student Cai Ni as the first author, and researcher Tu Xiongbing and researcher nongxiangqun as the co correspondents. The research was supported by the Ministry of Finance and the Ministry of agriculture and rural areas:the national modern agricultural industrial technology system (cars-34-07).
 
Original link:https://doi.org/10.3390/jof8070661