Recently, the Vegetable Research Institute published the title”Identification of Quantitative Trait Loci Controlling the Development of Prickles in Eggplant” in the international botany journal Frontiers in Plant Science (IF=5.75). forbid Genome Re-sequencing Analysis” research paper. This study carried out QTL mapping of the thorns on the main stem, leaves, petioles and sepals of eggplant, and provided a theoretical basis for cultivating thornless eggplant varieties.
Eggplant (Solanum melongena L.) is an important solanaceous vegetable crop in my country. The planting area has been expanding in recent years. As of 2019, the sown area of eggplant in the country reached 11.7 million mu (FAO). Eggplant stems, leaves, sepals and other parts may have a large number of thorns, but the thorn traits are not conducive to pruning, harvesting and other agricultural operations, and are unfavorable traits for agricultural production of eggplant. Therefore, research on eggplant thorn traits is carried out. Cultivating thornless eggplant varieties is of great significance.
In this study, thorny wild resources and less thorny cultivated inbred lines were used to construct an F2 eggplant genetic population for the parents, and the main stems, leaves, petioles and sepal thorns of eggplant were studied. The inheritance of these 4 quantitative traits was analyzed using the constructed 6-generation genetic population. The results showed that the best genetic models for eggplant stems, leaves, petioles, and sepal spines were in accordance with the additive dominant epistasis model of 2 pairs of major genes, and 1 pair of negative fully dominant major genes [[+]] were more additive dominant. Gene model, additive dominant epistasis polygene model and 2 pairs of major gene additive dominant epistasis models.
In this study, the parents and 100 F2 progeny were re-sequenced, and 2,989,312 and 1,369,910 high-quality SNP markers were obtained from the parents, respectively. Using these SNP markers, a high-density genetic map containing 278,891 Bin markers was constructed on the 12 chromosomes of eggplant. Using this genetic map, researchers performed QTL mapping for eggplant stems, leaves, petioles and sepal spines. The stem spines were mapped to 5 related QTLs on chromosomes 3, 4, 5, 6 and 8, leaf, petiole and sepal spines. One QTL was mapped on each of chromosomes 9, 1, and 12.
Based on the QLT positioning interval, the researchers found two WUSCHEL (WUS) related genes SEML_012g394430 and SEML_012g394440 as candidate genes for controlling the development of eggplant sepal spines. The researchers compared the differences between the two candidate genes in the parents and found that the SEML_012g394440 gene has a 22bp deletion in the sepal-free parent. This deletion region is located between the intron (18bp) and the exon (4bp). , The above deletions may lead to different splicing patterns and lead to differences in the phenotype of sepal spines. Further r-PCR results showed that the expression of SEML_012g394440 gene in sepal thorny parents and F2 progeny was significantly higher than that of sepal thornless parents and F2 progeny. The above research provides a theoretical basis for the inheritance and breeding of thorn-related traits of eggplant plants.
Assistant researcher Qian Zongwei and Zhang Bin of Vegetable Research Institute, Beijing Academy of Agriculture and Forestry Sciences are the co-first authors of this paper, and Cui Yanling extension researcher and Associate Researcher Li Dayong are the co-corresponding authors. At the same time, it has also been funded by the National Key Research and Development Program and the special funding for scientific and technological innovation capacity building of the Beijing Academy of Agriculture and Forestry Sciences.