Domestic animal stem cells not only have important scientific value in the field of basic research in life sciences, but also have broad application prospects in animal breeding and human disease models. However, compared with the research results of experimental animals and human stem cells, the key technology of livestock stem cell induction has not broken through. It is a difficult point in the world in this field, and it also restricts the breeding and clinical application of livestock stem cells.
This research adopts the TET-ON piggyBac system reprogramming technology, adopts the”8-factor induction combination” jointly designed by the project partner and the self-developed stem cell specific culture system, after five years, hundreds of times of key stem cell induction technology Explore and test the key factors of stem cell culture, and successfully obtained two sets of new bovine stem cells, bEPSCips and bEPSCES, established by somatic cells (bovine MEF) and early embryonic cells (bovine Blastocyst) with expanded pluripotency. Through in vivo and in vitro stem cell differentiation and chimerization experiments, it is proved that these two bEPSCs have the potential to differentiate into three germ layers. The biological location of their stem cells is in the 8-Cell~Morula stage of early embryos. Compared with similar studies at home and abroad, it fully shows the results of this study. Of new stem cells expand pluripotency.
Further research shows that the obtained two sets of expanded pluripotent bovine new stem cell bEPSCs can be used to rebuild”cloned embryos” through nuclear transfer SCNT technology, which can induce and regenerate the next generation of bovine new stem cells with a gene editing efficiency of 22.9%, which proves that the genetic characteristics of the expanded multipotent new bovine stem cells established in this study are stable, which is very important for the biological breeding and clinical application of stem cells in livestock. At present, Professor Li Xihe’s team has established a TOP genetic quality dairy cow stem cell line using this new technology, and has carried out the development and application of dairy cow biological breeding.
Proceedings of the National Academy of Sciences of the United States of America (PNAS) published online a new type of cattle led by the team of Professor Li Xihe from Inner Mongolia University and Secco Star Research Institute The latest achievements in stem cell research. This study reported for the first time the key technology and biological characteristics of the induction of bovine expanded potential stem cells (bEPSCs) that do not rely on exogenous genes. It is a milestone scientific and technological achievement in the research of herbivorous livestock stem cells.
This research is based on the joint construction of the State Key Laboratory of Grassland Animal Reproductive Regulation and Breeding by Inner Mongolia University, Professor Li Xihe from the Mongolian Plateau Animal Genetic Resources Research Center of the School of Life Sciences, and Professor Liu Pengtao from the Li Ka-shing School of Medicine of the University of Hong Kong as the co-corresponding authors , Dr. Zhao Lixia of Inner Mongolia University/Inner Mongolia SECCO Star Research Institute, Dr. Gao Xuefei of Southern Medical University and Dr. Zheng Yuxuan of Peking University are the co-first authors of the paper. The State Key Laboratory of Inner Mongolia University/School of Life Sciences, Professor Bao Siqin, Professor Tang Fuchou from the BIOPIC Center of the School of Life Sciences, Peking University, Professor Cao Guifang from the School of Animal Science and Technology of Inner Mongolia Agricultural University, and Inner Mongolia Secco Star Research Institute. The project is supported by the National Natural Science Foundation of China, the major science and technology project of Inner Mongolia Autonomous Region, and the first-class discipline construction funding of Inner Mongolia University.
link to the original text:https://doi.org/10.1073/pnas.2018505118