The main crops have entered the post-genomic era. Genotype identification is required for seed purity and variety identification, genome function research and molecular breeding. The establishment of a simple, fast and non-destructive continuous seed sampling technical process and a technical system for genotype identification can save planting costs and identification cycles, strengthen plant gene function research and improve the efficiency of molecular breeding.
Recently, Xia Zhengjun’s research group from Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences published a technical paper entitled”Rapid and Non-destructive Soybean Seed Continuous Sampling Technology and DNA Preparation” in”Acta Botany”, which is about seed purity and variety Identification and molecular design breeding provide technical support. This technology uses simple devices such as miniature electric drills and air pumps to ingeniously design a continuous and non-destructive drilling operation process for soybean seeds. When the drill bit reaches the endosperm part of the seed, place the thick end of the pipette tip under the drill bit with the left hand. The tip tube collects approximately 5.85–9.75 mg of endosperm tissue of soybean seeds. This technology is characterized by continuous sampling. The number of plant seed tissue samples that can be drilled per person per day is about 1500-2300 (equivalent to 4-6 384-well sample plates). Compared with the existing drilling method, Efficiency can be significantly improved. At the same time, the obtained samples do not need to be ground and can be directly used for downstream DNA extraction and genotype identification. This technology can be used in the research fields of variety identification, gene positioning, molecular assisted breeding and molecular design breeding of various plants.
Researcher Xia Zhengjun is the first and corresponding author of this article, and co-authors of this article such as Li Yuzhuo, Zhu Jinlong, Wu Hongyan, Xu Kun and Zhai Hong.
This research was funded by the key deployment projects of the Chinese Academy of Sciences, the Strategic Science and Technology Pilot Program of the Chinese Academy of Sciences (Class A), and the National Natural Science Foundation of China.
Article link https://www.chinbullbotany.com/CN/10.11983/CBB20095