Recently, the Crop Stress Tolerance Regulation and Improvement Innovation Team of the Institute of Biotechnology of the Chinese Academy of Agricultural Sciences revealed the differential mechanism of high temperature resistance between indica and japonica from the three-dimensional structure of chromatin, in order to study epigenetic regulation of important agronomic traits and Improving adversity resistance provides a new research perspective. Related research results were published in the international academic journal”BMC Biology” (BMC Biology).
According to experts, the three-dimensional structure of chromatin is highly correlated with genome function. At present, only a few eukaryotes have reported the impact of environmental changes on the three-dimensional structure of chromatin, but how the three-dimensional structure of crop chromatin responds to stress is still unclear. The study used three-dimensional spatial structure sequencing (HiC-seq), chromatin open sequencing (ATAC-seq) and transcriptome sequencing (RNA-seq) multi-omics analysis to reveal the three-dimensional three-dimensional chromatin of indica and japonica rice under high temperature stress. The dynamic change of structure emphasizes the importance of in-depth study of crop stress response mechanisms from the three-dimensional structure of chromatin.
The three-dimensional structure of chromatin in eukaryotes plays a vital role in the regulation of gene transcription and the control of various biological processes. This study revealed the differences in the three-dimensional structure of chromatin between indica and japonica under high temperature stress, and clarified the relationship between the three-dimensional structure of chromatin and gene transcription and chromatin accessibility. It provides a new research perspective for the analysis of rice epigenetic precision regulation mechanism and rice resistance improvement.
Researcher Gu Xiaofeng from the Institute of Biology is the corresponding author of the paper, and researcher Liang Zhe and Zhang Qian from the Institute of Biology are the first authors of the paper. This work has been funded by the National Major Project and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.
link to the original text:https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-021-00996-4