Sheep genetics and breeding team reveals the expression of key proteins in sheep sperm-egg fusion
By: Date: 2021-04-12 Categories: foodtechnology Tags: ,
  Recently, the mutton sheep genetics and breeding team systematically analyzed the expression, structure and function of Juno and Izumo1 genes and their encoded proteins, revealing the expression of key proteins in sheep sperm-egg fusion, and laying a foundation for further research on the regulatory mechanism of Juno and Izumo1 genes Theoretical basis. Related research results were published in the Journal of Animal Science and Biotechnology.
  Fertilization is a precise and highly coordinated physiological process in mammals, in which the fusion of sperm and egg is the most critical step. Two highly differentiated haploid germ cells, sperm and egg, merge to form a brand new fertilized egg and develop into an embryo. IZUMO1 and JUNO are the first ligand-receptor protein pairs found on the plasma membrane of sperm and egg. Their interaction is necessary for human and mouse sperm and egg recognition.
Sheep genetics and breeding team reveals the expression of key proteins in sheep sperm-egg fusionimage
  Figure 1 Identification and interaction between Izumo1 and Juno during sperm-egg fusion
  Researchers cloned the full-length DNA and cDNA sequences of the sheep Izumo1 and Juno genes, and discovered a new 35 bp fragment of the Izumo1 gene for the first time, and confirmed that the Izumo1 gene has 9 exons in small-tailed Han sheep instead of 8. A. Using single-cell transcriptome technology, the researchers analyzed the gene expression patterns in multi-lamb and single-lamb small-tailed Han sheep oocytes, granulosa cells and follicular membranes. Studies have found that the high expression of Juno gene in the ovary is mainly concentrated in oocytes. A SNP mutation in the gene is significantly related to littering traits. This SNP mutation can cause amino acid changes, which is important for littering traits. Negative Effects. According to bioinformatics analysis, this mutation makes the secondary structure and tertiary structure of the JUNO protein and the protein binding site change, which may lead to the change of protein function.
Sheep genetics and breeding team reveals the expression of key proteins in sheep sperm-egg fusionimage(1)
  Figure 2 Small-tail Han sheep JUNO and IZUMO1 protein tertiary structure prediction
   This research is supported by the National Natural Science Foundation of China Youth Fund Project.
   link to the original text:https://jasbsci.biomedcentral.com/articles/10.1186/s40104-021-00548-4