Recently, the team of Academician Kang Zhensheng of Northwest A&F University and the team of Professor Shunyuan Xiao of the University of Maryland published an online publication titled”AtSTP8, an endoplasmic reticulum-localized monosaccharide transporter from Arabidopsis, is recruited to the” in New Phytologist. extrahaustorial membrane during powdery mildew infection” research paper. The study found that the Arabidopsis sugar transporter AtSTP8, which is located in endoplasmic reticulum (ER), is recruited to the outer plasma membrane EHM of the haustorium during powdery mildew infection, and it may be involved in the transmembrane transport of host sugar to pathogens.
Living nutrient obligate parasitic fungi (rust fungus, powdery mildew, etc.) use suction devices to absorb sugar and other nutrients from host plants for their growth and development. In the process of bacterial infection, the sugar in the host cell first completes the transmembrane transport from the cytoplasm to the extrahaustorial matrix, and then is transported to the haustor by the sugar transporter located on the haustor membrane. However, at present, the process of host cell sugar transport across EHM and its mechanism of action are still unclear.
This study analyzed the subcellular localization of 14 members of the Arabidopsis STP (sugar transporter protein) family, and found that AtSTP2 and AtSTP6 are located in the vacuole membrane, AtSTP8 is located in the ER, and other family members are located in the plasma membrane. ; After inoculation with powdery mildew, only three members, AtSTP2, AtSTP6 and AtSTP8, which showed differential localization, were distributed around the haustor. The spatiotemporal expression characteristics and co-localization analysis (FM4-64/RPW8.2) confirmed that AtSTP8 was localized in EHM (Figure 1) . Yeast mutant complementation shows that AtSTP8 has a broad spectrum of sugar transport activity. In addition, overexpression of AtSTP8 can promote the susceptibility of Arabidopsis to powdery mildew and increase the hexose content of the leaves. Compared with the control, the AtSTP8 gene deletion mutant has no obvious change in disease resistance, and it may be functional Redundant genes. This study found for the first time a sugar transporter located in EHM, and the results lay the foundation for further analysis of the process of host cell sugar transport across EHM and its mechanism of action.
Professor Liu Jie from Northwest A&F University is the first author of the paper. Academician Kang Zhensheng of Northwest A&F University and Professor Xiao Shunyuan from the University of Maryland are the co-corresponding authors of the paper. The project has won the National Key Research and Development Program, the National Natural Science Foundation of China, etc. Project funding.
link to the original text:https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.17347