News from this website recently, Professor Wang Junjun from the College of Animal Science and Technology of China Agricultural University published an online publication entitled”Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages” the latest research results (https://doi.org/10.1080/19490976.2021.1903826).
Intrauterine Growth Restriction (IUGR) leads to high mortality in the early life of piglets and low growth performance in the later stages of life. Insufficient recognition of its intestinal development defects and nutrient utilization characteristics restrict the effectiveness of IUGR pig nutrition regulation The essential. To this end, Professor Wang Junjun’s research group has long been engaged in the study of IUGR pig intestinal structural and functional defects, inflammation occurrence and development, microbial differential colonization and nutritional intervention. Based on previous studies, it was found that the functional ingredient (CMFG) composed of milk fat globule membrane, galactooligosaccharide and fructooligosaccharide has a powerful probiotic effect. This study further systematically explored the effects of CMFG on the body’s intestinal barrier function, intestinal flora and inflammation. The effect of improving the response, and mediating intestinal microbial transfer and intestinal microenvironment reconstruction through co-cultivation, reveals the effect and mechanism of microbial transplantation to relieve intestinal inflammation.
First, based on a mouse colitis model induced by dextran sodium sulfate (DSS), the author found that pre-addition of CMFG significantly improved colon tissue morphology and intestinal tight junctions, and promoted colonic goblet cell proliferation and mucin MUC2 secretion (Figure 1), improve the expression of genes related to intestinal barrier function (Figure 2), inhibit the up-regulation of pro-inflammatory gene expression and excessive activation of the TLR4 signaling pathway, and inhibit intestinal epithelial cell apoptosis and colonic lamina propria pro-inflammatory macrophage polarization . At the same time, CMFG can promote the proliferation of short-chain fatty acid-producing bacteria (such as Ekmania, Lactobacillus and Rose bacteria), and improve the intestinal mucosal barrier by increasing the production of acetic acid and butyric acid, and inhibit intestinal epithelial cell apoptosis and oxidative damage. Play a probiotic effect in other ways, thereby alleviating colitis in mice (Figure 1). Therefore, gut microbes may play an indispensable role in CMFG alleviating colitis in mice.
In order to further verify the probiotic effect of the functional ingredient CMFG, the author used a mouse co-culture model and found that the intestinal flora after CMFG intervention can be successfully transplanted to the recipient mice, which restores the recipient mice to a certain extent The integrity of the colonic mucosal barrier (Figure 3), and the delivery of short-chain fatty acid producing bacteria such as Ekmania (Figure 4), enables the recipient mice to reproduce the intestinal inflammation phenotype.
This study used intestinal microbes and epithelial cells as the entry point. For the first time, it was found that the addition of functional ingredients such as oligosaccharides and milk fat globule membranes can increase the colonization of short-chain fatty acid-producing bacteria and promote the proliferation and adhesion of goblet cells. The secretion of protein MUC2 improves the intestinal microecology and mucosal barrier, reduces colonic lamina propria pro-inflammatory macrophages and improves the intestinal immune microenvironment, thereby alleviating intestinal inflammation; and after the establishment of the level of flora through polyculture, recipient mice The above phenotype was successfully reproduced and verified the mediating role of intestinal microbes in functional ingredients to improve intestinal homeostasis and alleviate inflammation, providing a new idea for the optimization of piglet nutrition.
Professor Wang Junjun from the School of Animal Science and Technology of our school is the corresponding author of the thesis, Liu Cong, a 2018 master’s degree graduate of the School of Animal Science and Technology (now a doctoral student at Leiden University Medical Center, the Netherlands), and a 2019 Ph.D. graduate, Huang Shimeng (now Jiangsu Province Agriculture) The Academy of Sciences and Washington University School of Medicine in St. Louis jointly trained postdoctoral fellows) as the co-first author of the paper. Teacher Zhang Bing from the School of Veterinary Medicine of our school provided strong support in the development of animal experiments, and teacher Zhao Jiangchao from the University of Arkansas in the United States gave guidance and help to the paper. This work was funded by the National Natural Science Foundation of China, the Beijing Municipal Natural Science Foundation of China, and the 111 Innovation and Intelligence Introduction Base.