Our scientists have developed a new type of biosensing technology for genetically modified detection
By: Date: 2021-04-09 Categories: foodtechnology Tags: ,
  Recently, the genetic safety evaluation and application team of the Institute of Biotechnology, Chinese Academy of Agricultural Sciences used surface plasmon resonance (SPR) to develop multiple, reproducible biosensing technologies for nucleic acid targets for the detection of multiple genetically modified elements Provides new enzyme-free and amplification-free technical support, which can realize rapid and efficient detection of genetically modified components. Relevant research results were published in the journal”Talanta”.
  Accurate, sensitive and rapid molecular diagnostic technology is the basis of biosafety testing, and biosensing technology provides a new direction and application strategy for this. Researchers used surface plasmon resonance technology to ingeniously design a multi-channel multi-target analysis strategy. When samples containing different targets are injected into the chip, different channels can output signals corresponding to the analyte, thereby realizing multiple detection. This strategy can realize the qualitative and quantitative analysis of the transgenic elements CaMV35s promoter, nos terminator and cry1A gene, and the detection accuracy is greatly improved. After optimization of the conditions, the detection line of the sensor technology for the above three targets can reach 0.1 nM. This method can not only realize rapid screening of transgenes, but also make chips that can be reused 100 times within 20 days, and maintain high detection performance.
Our scientists have developed a new type of biosensing technology for genetically modified detectionimage
   Legend:Multiple renewable surface plasmon resonance biosensors
   The research was funded by the major project for the cultivation of new varieties of genetically modified organisms and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences. (Correspondent Cui Yan)
   link to the original text:
  https://www.sciencedirect.com/science/article/abs/pii/S0039914021002824
  https://www.sciencedirect.com/science/article/abs/pii/S0956566320309283
  https://link.springer.com/article/10.1007%2Fs00604-020-04511-4
  https://doi.org/10.1039/D1AN00313E