Flammulina velutipes polysaccharide is the main functional component with multiple biological activities in Flammulina velutipes. After a complicated production process and various factors, the quality of the products is uneven, and it is difficult to distinguish between good and bad. With the diversified development of polysaccharide products, how to identify the quality of polysaccharide products has become an urgent concern. However, the current research on the quality standards of Flammulina velutipes polysaccharide products is relatively rare, and there is no established unified standard and scientific and effective identification method.
In response to the above problems, the team of Professor Qiuhui Hu from Nanjing University of Finance and Economics established the scanning electron microscope, Fourier transform infrared spectroscopy and high performance liquid chromatography multi-dimensional fingerprint spectrum of Flammulina velutipes polysaccharide, and measured them The IC25 value of the inhibitory effect of different sources of Flammulina velutipes polysaccharides on human liver cancer HepG-2 cells was established by multiple linear regression analysis to explore the relationship between the spectrum and the effect. The results showed that the apparent characteristics of 20 batches of Flammulina velutipes polysaccharides from different sources were highly similar. Infrared spectroscopy and high performance liquid chromatography fingerprints combined with chemometric methods proved that Flammulina velutipes polysaccharides had specific standard fingerprint characteristics and successfully identified 3 species. Flammulina velutipes polysaccharide of unknown origin. At the same time, HPLC fingerprint can further distinguish white and yellow Flammulina velutipes polysaccharides. Mannose (+), rhamnose (-), galactose (-), and xylose (-) have a significant effect on the inhibitory effect of HepG2 cells (P<0.05). The above research results are named as"Multiple fingerprint and fingerprint-activity relatio nship for quality assessment of polysaccharides from Flammulina velutipes” was published in the journal Food and Chemical Toxicology. Master student Dong Yutong of our school is the first author, and Professor Hu Qiuhui is the corresponding author.
Flammulina velutipes infrared spectroscopy fingerprint analysis
Flammulina velutipes HPLC fingerprint analysis