Continuous cropping leads to the decline of the anti-disease function of the bacteria colonizing the hyphae of the pathogenic bacteria
By: Date: 2021-04-12 Categories: foodtechnology Tags: ,
   Recently, the Research Group of Soil-borne Disease Biological Control of the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences and the Netherlands Institute of Ecology have published research results in Applied Soil Ecology. The study revealed the relationship between different farming systems and the composition and function of bacterial populations colonizing the hyphae of Fusarium wilt fungus. It was found that the composition of bacterial populations colonizing the hyphae of Fusarium wilt fungus and the abundance of dominant bacteria were affected by continuous cucumber cropping and pathogen inoculation. Significant changes have occurred in the temperature, and the disease suppression function of bacteria has declined. The results of the study provide a new explanation for the aggravation of cucumber fusarium wilt under continuous cropping conditions, and provide a new idea for the management of fusarium wilt.
  Soil is a complex ecosystem. The tiny area where the fungal hyphae in the soil is in contact with the soil is the forefront of bacterial antagonistic fungi in the soil. The community composition and functions of the bacteria colonizing the hyphae vary with the changes of fungal species. At present, there is a lack of research on the colonization of bacteria around the hyphae of plant pathogenic fungi under different farming systems at home and abroad. To clarify the relationship between the population composition and function of the colonizing bacteria around the cucumber Fusarium wilt fungus hyphae and the farming system, to reveal from a new perspective the mechanism of soil disease suppression function decline under continuous cropping conditions, to discover new biological control factors and find new control The control strategy is of great significance.
   The study investigated the effects of continuous cropping and non-continuous cropping, inoculation with Fusarium wilt, and long-term continuous cropping after inoculation with Fusarium wilt on the colonizing bacteria around the hyphae of Fusarium wilt through the establishment of artificial sickbeds and greenhouse pot experiments. First, collect the rhizosphere soil of different treatment cucumbers before planting and different growth periods (2 weeks and 6 weeks after emergence), and use the hyphae trap method to obtain the colonizing bacterial suspension on the hyphae, and then use the Illumina sequencing method to analyze the different treatment Fusarium wilt bacteria The population diversity of colonizing bacteria around the hyphae, the composition of bacterial populations, and the relative abundance of bacteria. It was found that cucumber growth time and continuous cropping are the main factors affecting the changes in the population composition and abundance of colonizing bacteria around the hyphae of Fusarium wilt, followed by Fusarium wilt bacteria inoculation. Fusarium wilt has a certain selective effect on bacteria in the soil. The dominant bacteria colonizing around the hyphae of Fusarium wilt are Rhizobium and Achromobacter, and the inoculation of Fusarium wilt caused significant changes in the population abundance of these two dominant bacteria. The population abundance of Rhizobium bacteria decreased, and the abundance of Achromobacter bacteria increased. Based on the changes in the abundance of these two genera of bacteria, two bacterial combinations were designed, and the cultivable bacteria were used to determine the anti-disease function of different bacterial combinations. It was found that the decrease in the abundance of Rhizobium bacteria and the increase in the abundance of Achromobacter bacteria were caused The inhibitory effect of bacteria on fusarium wilt decreased. The results of the study revealed that continuous cropping and the introduction of Fusarium wilt are important reasons for the decline of the bacterial community in the soil. It was found that the rhizobia could play a role in disease prevention as a new biocontrol factor, and provided new ideas for the prevention and control of cucumber fusarium wilt. Ideas.
  Master students Sun Ruiliang and Jing Yuling are the first authors of the thesis. Associate researcher Guo Rongjun, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, is the corresponding author of the paper. The research was funded by the China Scholarship Council Scholarship Fund, Modern Agricultural Industrial Technology System and other projects.
   related paper information:https://doi.org/10.1016/j.apsoil.2021.103977
Continuous cropping leads to the decline of the anti-disease function of the bacteria colonizing the hyphae of the pathogenic bacteriaimage
Continuous cropping leads to the decline of the anti-disease function of the bacteria colonizing the hyphae of the pathogenic bacteriaimage(1)