Recently, the Crop Transgenic Technology and Application Innovation Team of the Institute of Crop Science, Chinese Academy of Agricultural Sciences used CRISPR-Cas9 technology combined with an efficient millet genetic transformation platform to edit the millet SiMTL gene to obtain a millet haploid induction line, and successfully through selfing and For the first time, a millet haploid was obtained by hybridization. Relevant results were published in”Plant Biotechnology Journal” on March 22.
According to Associate Researcher Sui Yi, millet is one of the important crops in my country. It has the characteristics of tolerance to barrenness and high light efficiency. It is mainly planted in arid and semi-arid areas, with a planting area of more than 20 million mu. Millet is full of nutrition, especially rich in vitamins and trace elements necessary for the human body, and has important nutritional value. As people’s health needs and dietary structure change, the demand for millet, a miscellaneous grain, is increasing. Therefore, new varieties with better quality, better taste, wide adaptability and stronger resistance are the development of future breeding. direction. Compared with traditional breeding, haploid induction and doubling technology can make the genetic material homozygous for one generation, greatly improving the breeding schedule and efficiency. However, the study of millet haplotypes is still blank, and the introduction of haplotypes is one of the future trends.
Researchers used CRISPR-Cas9 technology to knock out the SiMTL gene in millet, and first created a haploid induction line. The frequency of haploids in the offspring of the induction line was used to estimate its inducibility, and flow cytometry was used to confirm that the haploid occurrence frequency of selfed offspring was 2.7%. On this basis, using the sterile line used in production as the female parent and the induction line to cross, the haploid of the hybrid offspring was identified and found that the haploid induction efficiency reached 2.6%. This research has laid a solid foundation for the establishment of a millet double-haploid sports breeding platform, and is of great significance for the following to further increase the frequency of haploid induction and speed up the process of millet breeding.
Cheng Zixiang, a doctoral student in the Department of Composition, Sun Yao, a doctoral student jointly trained by the Institute of Composition and China Agricultural University, are the co-first authors of the paper, and Associate Researcher Sui Yi is the co-corresponding author. Researcher Diao Xianmin, Wisdom Researcher, Li Xinhai, and Guo Yan from China Agricultural University also participated in this research. The research was funded by the National Key Research and Development Program and the Innovation Project of the Chinese Academy of Agricultural Sciences.
Link to the paper:https://doi.org/10.1111/pbi.13584