Recently, the Smart Animal Husbandry Innovation Team of the Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences has made a new breakthrough in the research of biological sensing technology, providing a new method for the development and application of non-specific DNA enzyme biosensors. Related research results are published in”Sensors and Actuators:Chemistry (Sensors and Actuators B:Chemical)”.
According to the team’s chief researcher, Hai Kumamoto, with the development of modern society, the application of biosensors has become more and more extensive, but in the detection of metal ions, especially large Some heavy metal ions lack deoxyribozymes with good selectivity and high sensitivity. The researchers used the common heavy metal cadmium ion (Cd2+) as the detection target, innovatively used non-specific DNAzymes that are easily interfered by mercury ions (Hg2+) and lead ions (Pb2+) to construct a biosensor array, and realized cadmium ions through regression models. Qualitative and quantitative analysis, and the detection accuracy is greatly improved. Under optimized conditions, the detection range of the biosensor array for cadmium ions covers 5.0×10-2～1.0×103 nanomoles, and the maximum detection limit reaches 3.4×10-2 nanomoles. This research provides a new method for the development of more and more high-precision biosensors.
The research is supported by the National Key R&D Program and Guangdong Province Key Field Research Program.
link to the original text:https://doi.org/10.1016/j.snb.2020.129139