Li Yi’s group from Peking University School of Life Sciences reveals the molecular mechanism of rice auxin response factors (OsARFs) differentially regulating rice resistance to dwarf virus (RDV)
By: Date: 2021-03-27 Categories: foodtechnology Tags: ,
  Recently, PLOS Pathogens magazine published a report entitled”Auxin response factors (ARFs) differentiallyregulate rice” from the research group of Professor Li Yi from the School of Life Sciences, Peking University. antiviral immune response against rice dwarf virus” research paper. Reveals the molecular mechanism of rice auxin response factors (OsARFs) differentially regulating rice resistance to dwarf virus (RDV).

  Rice dwarf virus (Rice dwarf virus, RDV) is a virus transmitted by leafhoppers that can cause large-scale rice susceptibility and severely reduce production. Rice plants infected with RDV are significantly dwarfed, have more divisions, and do not head or half head. The previous research results of the research group showed that the coat protein P2 encoded by RDV competes with the auxin receptor OsTIR1 to bind to OsIAA10, inhibits the degradation of OsIAA10 protein through the 26S proteasome, and promotes the accumulation of OsIAA10 protein, thereby affecting the response and response of the auxin pathway The transcription of downstream genes promotes the infection of RDV and the formation of disease symptoms (PLOS Pathogens, 2016). Although this reveals to a certain extent the relationship between the auxin signaling pathway and RDV infection, the specific molecular mechanism of disease resistance downstream of OsIAA10 is still unclear. Through molecular, biochemical and genetic methods, researchers found that after RDV infection, the content of auxin in rice increased, and at the same time, exogenous application of auxin promoted the degradation of OsIAA10 protein, which relieved the inhibition of OsIAA10 on its bound OsARF transcription factor. Enhance rice resistance to viruses. In-depth studies have found that IAA10 can interact with five ARF transcription factors. Among them, ARF12 and 16 positively regulate rice resistance to viruses, while ARF11 negatively regulates rice resistance to viruses. Defensive genes such as OsWRKY13, a downstream gene of ARF12, are involved in resisting RDV infection. In summary, this study reveals the important role of OsIAA10-OsARF12-OsWRKY13-mediated signal pathway in rice anti-virus defense and virus anti-virus defense, and provides a reference for rice disease resistance molecular breeding. At the same time, the study also found that OsIAA10-OsARF11 in rice negatively regulates rice disease resistance to viruses and the ARF antiviral pathway that does not rely on IAA10.

Li Yi’s group from Peking University School of Life Sciences reveals the molecular mechanism of rice auxin response factors (OsARFs) differentially regulating rice resistance to dwarf virus (RDV)image
  OsIAA10-OsARF12-OsWRKY13 mediates the molecular mechanism of RDV resistance

   Peking University graduated PhD student Qin Qingqing and PhD student Li Guangyao are the co-first authors of the paper. Academician Xu Zhihong of Peking University participated in the research, Beijing Professor Li Yi from the University, Professor Wang Haiyang from South China Agricultural University, and Dr. Zhirui Yang, a postdoctoral fellow from Peking University, are the corresponding authors of the paper. This research has been strongly supported by the National Rice Industry System Project, the National Natural Science Foundation of China, the National Genetically Modified Special Project and the NSFC-Guangdong Joint Fund.