Li Shigui’s team from Sichuan Agricultural University identified an excellent natural allele of TAW1, a rice grain number gene per panicle
By: Date: 2021-03-26 Categories: foodtechnology Tags: ,
  Recently, the team of Professor Li Shigui from Sichuan Agricultural University published an online publication titled”TAW1 Natural Alleles Helps to Improve Rice Ears and Grains” in the TOP journal”The Crop Journal” (The Crop Journal) in District 1 of the Chinese Academy of Sciences.”Number and yield” (A natural allele of TAW1contributes to high grain number and grain yield in rice). The research lasted twelve years from construction to discovery to publication today. In this study, the author identified a cloned natural allele of TAW1 by QTL mapping, which has important value for breeding.
  The number of grains per panicle is one of the factors that directly determines the yield of rice. The identification of genes or QTLs related to the number of grains per panicle is of great significance for improving rice yield. At present, a large number of genes related to grain number per ear have been cloned, but most of the genes are identified through mutant materials, which are difficult to directly use in breeding. Therefore, it is of great significance to discover the natural excellent alleles of genes related to grain number per ear.
   In this study, the authors used the backbone parent Shuhui 498 (R498) of heavy panicle hybrid rice as the recipient, and a longer panicle indica rice variety WY11327 was used as the donor to construct a set of chromosome segment substitution lines, and one was identified The substitution line F771 with a spike length of 38 cm. Through the construction of segregation and mapping population, genetic analysis, QTL-seq mapping and candidate gene analysis, it was determined that the candidate gene of qPLN10 (Panicle Length and Grain Number 10) located on chromosome 10 was the cloned ear grain number gene TAW1. The near-isogenic line NIL-TAW1 isolated from the gene was further screened by molecular markers. Compared with R498, the ear length and grain number of NIL-TAW1 increased significantly, and the final yield per plant increased significantly (Figure 1), indicating the gene’s effectiveness Natural variation has important breeding value. Haplotype analysis found that the excellent haplotype Hap13 mainly exists in aus resources, but the proportion is very small in indica and japonica rice (Figure 2). It is speculated that the excellent haplotype may be derived from aus, which is useful for guiding the improvement of indica and japonica rice. The traits of panicle length and grain number per panicle are of great significance to improve rice yield.
  Dr. Yuan Hua, master students Xu Zhengyan and Tan Xueqin are the co-first authors of this article, and Professor Ma Bingtian and Dr. Chen Weilan are the co-corresponding authors. This research was funded by the cultivation of indica rice varieties (2016ZX08001004-002) and the national key research and development program (2016YFD0100406), a sub-project of the national major special project of genetically modified science and technology.