On February 2, Professor Zhu Yiyong’s team from the School of Resources and Environmental Sciences of Nanjing Agricultural University made new progress in the research of rice cell membrane proton pumps. Studies have found that overexpression of the cell membrane proton pump OSA1 in rice can not only promote the absorption of nitrogen and other nutrients by the roots, but also increase the stomata opening of the leaves, thereby increasing the nitrogen use efficiency and photosynthesis rate of rice, and significantly increase Increased the yield of rice. This research provides a theoretical basis for the efficient use of crop nutrients, as well as new ideas for reducing environmental pollution caused by excessive fertilization and mitigating the greenhouse effect.
cell membrane proton pump, also known as cell membrane proton ATPase (PM H+-ATPase), is a ubiquitous enzyme in cells, which uses the energy generated by ATP hydrolysis The hydrogen ions are expelled from the cell and provide membrane potential and proton driving force for the transportation of nutrients inside and outside the cell membrane. The proton pump has a powerful physiological function in the plant. This study first established that the proton pump is involved in the absorption and regulation of ammonium nitrogen in rice, and it is clear that overexpression of the proton pump gene OSA1 can promote the acquisition and assimilation of ammonium by rice. At the same time, because the proton pump also participates in the signal transduction of the stomatal response to light, overexpression of the proton pump gene OSA1 can also increase the stomata opening of the leaves, enhance the absorption of carbon dioxide by the leaves, and promote the rate of photosynthesis. Field experiments showed that the yield of OSA1 overexpression rice was significantly increased, and the nitrogen utilization efficiency was also greatly improved; while the OSA1 knockout rice mutant lines showed the opposite phenotype to the overexpression rice. Transcriptome sequencing results showed that overexpression of OSA1 significantly increased the transcription level of important genes related to carbon and nitrogen metabolism in plants. In addition, this study also found that OSA1 overexpression rice can simultaneously increase the absorption of other nutrients (such as phosphorus and potassium), providing a theoretical basis for further exploring the mechanism of rice nutrient absorption.
The research results were published online in Nature Communications. Dr. Zhang Maoxing (Postdoctoral Fellow, Nagoya University), School of Resources and Environmental Sciences, Nanjing Agricultural University, Researcher Wang Yan, Peking University, Associate Professor Chen Xi from the School of Life Sciences, Nanjing Agricultural University, Dr. Xu Feiyun, Nanjing Agricultural University (now a postdoctoral fellow at Fujian Agriculture and Forestry University), this paper is the joint first The authors, Professor Yiyong Zhu from Nanjing Agricultural University and Professor Toshinori Kinoshita from Nagoya University, Japan are the co-corresponding authors of the paper. Teachers and students from the research groups of Nanjing Agricultural University, Shanghai Jiaotong University, Hangzhou Normal University, Anhui University of Science and Technology, Shanxi Agricultural University, Chinese Academy of Agricultural Sciences, Nagoya University and Chubu University in Japan, Liebig University in Germany and other related cooperative units participated in part of the work of the paper. This research has been awarded the National Key Research and Development Project (2017YFD0200200/0200206), the National Natural Science Foundation of China (NSFC31471937), the Low-Carbon Technology Research and Development Project of the Ministry of Science and Technology of Japan (JPMJAL1011), the Japanese Advanced Science Research Project (15H05956, 20H05687) and Anhui Province Supported by the Natural Science Foundation of China (1608085MC59).
link to the original text:https://www.nature.com/articles/s41467-021-20964-4