The result of the collaboration between the medicinal and food dual-use resources research team of the National Edible Fungus Processing Technology Research and Development Center and the team of researcher Zhang Yan,”Mechanism of intestinal flora and proteomics in regulating immune function of Durio Zibethinus rind polysaccharide” was recently published in the Journal of the Chinese Academy of Sciences JCR 2 Oxidative Medicine and Cellular Longevity” (IF:5.834) magazine. This research was supported by the Chinese Academy of Medical Sciences’ Digestive Tract Microecological Pharmacology and Toxicology Innovation Unit and the Chinese Academy of Medical Sciences’ Medical and Health Technology Innovation Project (2019-I2M-5-055) project support.
Durian is the fruit of Durio zibethinus Murr (Durio zibethinus Murr), an evergreen tree in the genus Durian of the Ceiba family. The flesh is sweet and warm, and has anti-inflammatory activity. Durian peel is the peel of durian. Current research is mainly about surface chemistry and adsorption function, but there are relatively few studies on its functional activity. As biological macromolecules, polysaccharides play an important role in regulating intestinal flora, treating constipation, and immune regulation. The early laboratory research on durian skin polysaccharides also found similar findings. The biopolysaccharide has the effects of enhancing the body’s immunity, regulating the intestinal flora, optimizing the intestinal environment, and improving the symptoms of constipation. To this end, this article has conducted a detailed and in-depth study on the mechanism of durian skin polysaccharides to enhance the body’s immunity.
This study uses cyclophosphamide (CTX) administration to establish an immunosuppressive mouse model. Through proteomics and intestinal flora studies, the effect of durian skin polysaccharide on the immune function of immunosuppressed mice is evaluated.
The results show:Compared with the model group, the durian skin polysaccharide high-dose group (200 mg/kg) can significantly increase the thymus and spleen index, improve the spleen tissue structure; significantly increase the cytokine interleukin-2 (IL-2) , Interleukin-4 (IL-4), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) content; increase serum acid phosphatase (ACP), lactate dehydrogenase (LDH), ultra Oxide dismutase (SOD) and total antioxidant capacity (T-AOC) activity; increase the activity of catalase (CAT) in the liver, reduce the content of malondialdehyde (MDA), and enhance the immune activity of immunosuppressed model mice. Proteomics studies have shown that compared with the model group, the administration group can significantly up-regulate the expression of low-affinity immunoglobulin Fc receptor III (FcγRIII), signal converter and transcription activator 1 (STAT1), and participate in Autoimmune thyroid disease , Staphylococcus aureus infection and regulation of metabolic pathways such as NF-κB signaling pathway. Intestinal microbial studies showed that compared with the model group, the SCFAs content in the administration group increased, the relative abundance of the beneficial bacteria Akkermansia, Bacteroides, and Paraprevotella in the intestinal flora increased, while the relative abundance of Ruminococcus and Oscillospira decreased. These results indicate that durian skin polysaccharides can have a beneficial effect on immune regulation by regulating protein expression and improving flora composition.
The team has conducted continuous research on the immune regulation activity of polysaccharides, and the results are published in Food Research International (IF5.978), the article link is https://doi.org/10.1016/j.foodres.2020.109316; Oxidative Medicine and Cellular Longevity (IF5.834), link to the article https://doi.org/10.1155/2020/1603735.