The result was published in the”Food and Chemical Toxicology” magazine (IF:4.679, JCR TOP Q2 area) with the title”Glucose absorption regulation and mechanism of the compounds in Lilium lancifolium Thunb on Caco-2 cells”
The chemical components of Lilium lancifolium Thunb mainly include saponins, alkaloids, flavonoids, phenolic glycerolipids and polysaccharides, etc. The pharmacological activities include antibacterial, hypolipidemic, anti-inflammatory and antioxidant Wait. Our research group isolated compounds from Lilium lily flowers:kaempferol, caffeic acid and quercetin-3-O-β-D-galactoside.
Literature research found that there are many studies on the pharmacological activities of kaempferol, caffeic acid and quercetin-3-O-β-D-galactoside, such as anti-inflammatory, regulating blood lipid, anti-oxidation and anti-depression. But there are no reports of kaempferol, caffeic acid and quercetin-3-O-β-D-galactoside based on the glucose absorption of Caco-2 cells. Therefore, in this paper, Caco-2 cells are used as a model to study the regulation and mechanism of the three compounds isolated from Lilium lily flowers on the glucose uptake of Caco-2 cells.
The experimental results show that the cultured Caco-2 cells can naturally differentiate into mature small intestine cells with similar morphology and biochemical characteristics. The Caco-2 cells at this time can be used as an in vitro model for studying small intestinal absorption, and the experiment can be carried out after culturing to 15-16 days.
Caffeic acid and quercetin-3-O-β-D-galactoside can promote the absorption of glucose by Caco-2 cells. Kaempferol had no significant effect on the glucose content in the supernatant of Caco-2 cells.
Fluorescent D-glucose homolog (2-NBDG) is a tracer probe to detect the influence of compounds on the glucose uptake and consumption of Caco-2 cells. Take pictures with an inverted fluorescence microscope. The uptake of 2-NBDG is proportional to the fluorescence intensity. This is a measure of the sugar uptake capacity of Caco-2.
On this basis, we will further explore the signaling pathways SGLT1 and GLUT2 related to glucose uptake in Caco-2 cells. Explain the mechanism of caffeic acid and quercetin-3-O-β-D-galactoside in promoting glucose absorption in Caco-2 cells.
The above studies have found that caffeic acid and quercetin-3-O-β-D-galactoside promote the transcription of SGLT1 and GLUT2 mRNA and the expression of their protein, thereby promoting the glucose absorption of Caco-2 cells.
The team has conducted a series of studies on glucose digestion, absorption and transport, and formed a mature research strategy. The research results have been published in Future Medicinal Chemistry (https://doi.org/10.4155/fmc-2018-0515 ); International Journal of Biological Macromolecules (https://doi.org/10.1016/j.ijbiomac.2020.05.042); Food Science and Human Wellness (https://doi.org/10.1016/j. fshw.2020.02.002; https://doi.org/10.1016/j.fshw.2020.04.005); Biomedicine & Pharmacotherapy (https://doi.org/10.1016/j.biopha.2020.109952); Food and Chemical Toxicology (Https://doi.org/10.1016/j.fct.2019.110806) and other magazines.