Recently, Xiaofeng Gu’s team and collaborators from the Institute of Biotechnology, Chinese Academy of Agricultural Sciences revealed a new mechanism for epigenetic regulation of cell cycle and DNA damage in rice, which provides an opportunity for studying epigenetic regulation of important agronomic traits of crops and improving stress resistance New ways and genes. The relevant results were published in the international academic journal”The Plant Cell (The Plant Cell)”.
During the growth and development of crops, abiotic stresses such as high temperature and drought can inhibit cell proliferation. Concentrating energy for stress resistance often leads to varying degrees of crop yield reduction. In the process of cell division, both genetic information and epigenetic information need to be transferred from the parent cell to the daughter cell. Therefore, the identification of regulatory genes and networks in them is expected to provide genetic resources and resources for improving crop yield and stress resistance. Theoretical basis. We have identified the yeast CTF4 homologous protein DRW1 in rice. DRW1 recruits members of the Polycomb family (PcG) complex to maintain histone H3 27th lysine trimethylation (H3K27me3) modification level in dividing cells. Participate in DNA replication in the cell cycle. In addition, DRW1 interacts with the DNA helicase complex member GINS and DNA polymerase DNAP to regulate DNA replication and damage repair in the S phase of the cell cycle. Compared with yeast and mammals, we found that rice DRW1 is not only involved in affecting DNA helicase activity, DNA replication and damage repair in the S phase of the cell cycle, but also specifically affecting the transmission of epigenetic information in the cell cycle, indicating that DRW1 is in the evolutionary process It presents a conservative biological function, and differentiates a unique apparent regulatory mechanism. In addition, compared with the Arabidopsis CTF4 homologous protein (EOL1), it is found that although both rice DRW1 and Arabidopsis EOL1 are expressed in the tissues with vigorous dividing cells, they can directly interact with members of the PcG complex and jointly promote H3K27me3 Modification levels are deposited, but the biological processes downstream of the regulation are significantly different, resulting in obvious phenotypes after the DRW1 mutation in rice. Rice DRW1 is involved in the regulation of cell cycle-related genes KRPs, while KRPs in Arabidopsis is not affected by EOL1. In addition, the Arabidopsis EOL1 mutation did not affect DNA damage, further indicating that the homologous proteins DRW1 and EOL1 of CTF4 have functional differentiation in mono and dicot plants.
The above studies show that the biology of CTF4 in yeast, Arabidopsis and rice has both functional conservation and species differentiation specificity, which provides a theoretical basis for subsequent studies on the evolution of CTF4 homologous protein species. In addition, the drw1 mutation showed obvious dwarf short phenotypes (including reduced plant height, smaller seeds, reduced thousand-grain weight, etc.). Further study of the function of DRW1 provides a way for the subsequent genetic improvement of rice growth and development, yield and stress resistance related traits. Clues and application prospects.
Researcher Gu Xiaofeng from the Institute of Biology, Professor Wu Changyin of Huazhong Agricultural University, and Researcher Yi Keke from the Institute of Regional Planning are the corresponding authors of the paper. This work was funded by the National Major Project and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.
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