(2) Ecological benefits
The key technologies such as artificial breeding of freshwater bass and feed domestication have made many major breakthroughs in production and application. The breakthrough of large-scale seedling breeding technology has met market demand. Variety improvement and new variety research and development have provided guarantee for the source of sea bass fry and industrial development. Compound feed replaces the cultivation method of chilled feed, reduces the consumption of marine fishery resources, and effectively reduces the pollution of aquaculture tail water, with significant ecological benefits.
(3) Social Benefits
Freshwater bass has become an important economic fish in freshwater in our country. Its delicious meat and no intermuscular spines are very popular with consumers. In 2004, the Ministry of Agriculture listed perch as a pollution-free food in relevant industry standards. After analyzing the polyunsaturated fatty acid composition of seabass, the British health department also included seabass as a pollution-free food and became a source of high-quality protein in the diet of residents. While enriching consumers’ food baskets and satisfying consumers’ demand for high-quality protein, the perch industry has also played a vital role in increasing farmers’ income and solving farmers’ employment issues. In 2018, my country’s perch production reached 432,100 tons, accounting for 1.70%of the country’s total freshwater aquaculture fish production. The aquaculture area covers 26 provinces (municipalities and autonomous regions) with an output value of over 10 billion yuan. Radiation drives circulation, processing, animal protection, and feed. Other production links have made important contributions to solving the employment of farmers, increasing the income of cooperative farmers, and promoting the increase of people’s income and prosperity.
In recent years, the common pathogens of largemouth bass farming in my country mainly include viruses, bacteria and parasites.
largemouth bass viral diseases mainly include viral ulcer disease, spleen and kidney necrosis and rhabdovirus disease. Viral ulcer disease is mainly caused by iridescent virus infection of the genus Ranavirus. The water temperature of the disease is 25~30℃, which mainly harms adult fish. The course of disease is long and fulminant deaths generally do not occur. Spleen and kidney necrosis is caused by the iridescent virus of the swollen virus genus. It is most likely to spread when the water temperature is 28~30℃, and it mainly harms adult fish. The infection often shows fulminant death. Rhabdovirus disease is caused by rhabdovirus infection. It is most susceptible to disease when the water temperature is 25~28℃. Especially when the water temperature suddenly rises or falls, it is easy to cause the disease. The disease mainly harms the fry, spreads fast, and kills. high. The virus disease of largemouth bass is basically similar to that of mandarin fish. There is no effective treatment method at present, but the prevention and control measures and countermeasures of virus disease in mandarin fish farming can be used for reference.
Bacterial diseases of largemouth bass mainly include Flavobacterium columnar disease, Aeromonas disease, Nocardia disease, etc. Flavobacterium columna is caused by the infection of Flavobacterium columna. The water temperature of the disease is 25-28℃, which can be harmful to fish species and adult fish. Largemouth bass cultured in high density or cages is more susceptible to disease. April to May is the period of high incidence of disease. , The mortality rate can reach 60%. Enteritis and hemorrhagic disease is caused by Aeromonas infection. The water temperature of the disease is about 30°C. The infection can be caused at all stages of breeding. It is mainly related to the intake of unclean food. Once the infection occurs, it can lead to fulminant death and high mortality. Nocardiosis is caused by Nocardia amberjack infection. The water temperature of the disease is 25-28℃, which mainly harms adult fish. Nocardiosis is a chronic disease with a long incubation period and is difficult to be detected. Therefore, its morbidity and mortality are both Higher, and seriously affect the commercial value of adult fish. Except for nocardia disease, other bacterial diseases can be controlled by external consumption and internal administration; in addition, the prevention and control of parasitic diseases should be strengthened to reduce secondary bacterial infections.
largemouth bass parasitic diseases mainly include trichosanthes, oblique tube worms, dactylus ringworms, branchworms, anchor head worms, etc. Trichoderma, oblique tube worm, dactylus ringworm, etc. are more harmful to seedlings, which can cause gill filament damage and cause a large number of seedling deaths. The branchworms and anchor heads mainly harm the adult fish. They attach to the host’s body surface, gills, and mouth, etc., and can cause death when they are severely parasitic. The prevention and control of parasitic diseases should not only diagnose the pathogen and use symptomatic drugs, but also regulate the water quality of the breeding pond.
With the advancement of largemouth bass feed preparation technology and the enhancement of environmental protection awareness, the proportion of compound feed used in largemouth bass breeding has increased in the past three years. The range is increased, and the use of chilled fish feed is correspondingly reduced. The production and sales volume of compound feed increased from 30,000-40,000 tons in 2015 to about 250,000 tons in 2019, and the output value was close to 3 billion yuan. In 2019, the amount of compound feed reached 55-60%of the market’s available capacity, and it is expected to show a steady upward trend in the future. At present, there are more than 30 manufacturers involved in the production of special compound feed for largemouth bass in the country, mainly distributed in the main producing areas of largemouth bass, namely the Pearl River Delta and the Yangtze River Delta. Among them, the amount of compound feed for largemouth bass produced in the Pearl River Delta region is about 180,000 tons, accounting for about 72%of the national output; the amount produced in the Yangtze River Delta region is about 50,000 tons, accounting for about 20%of the national output ; The output in other regions is about 20,000 tons, accounting for about 8%of the national output. According to the ranking of enterprises (or groups), there are only 1-2 companies with an annual production and sales of more than 40,000 tons of special compound feed for largemouth bass; about 4-5 companies with an annual production and sales of more than 20,000 tons; and most The annual production and sales volume of the company is about 5,000 tons.
(5) Circulation and processing
There are currently about 120 freshwater perch circulation companies, mainly in the Pearl River Delta. In 2017, the output of freshwater bass in Guangdong Province was 296,600 tons, accounting for 64.91%of the country’s total output, and the circulation ratio was over 90%. Calculated on the basis of 90%of the circulation, the transaction volume of Guangdong freshwater bass in 2017 was 266,900 tons, with a transaction volume of about 8 billion yuan. In 2018, the output of freshwater bass in Guangdong Province was 258,400 tons, accounting for 59.81%of the national total, with a transaction volume of 232,500 tons and a transaction volume of about 7 billion yuan. It is estimated that the trading volume of Guangdong freshwater bass in 2019 will reach 250,000 tons, with a trading volume of about 7.5 billion yuan. In 2017, the output of freshwater perch in Hubei Province was 0.99 million tons, accounting for 2.17%of the total output of the country. There were about 20 aquatic products circulation companies. Calculated based on the proportion of circulation accounting for 90%of the total output, the transaction volume of freshwater perch in Hubei in 2017 was 0.89 million tons. , The transaction volume is 270 million yuan. In 2018, the output of freshwater bass in Hubei Province was 12,300 tons, accounting for 2.85%of the country’s total output. The transaction volume was about 11,000 tons, and the transaction volume was about 330 million yuan. It is estimated that the trading volume of Hubei freshwater perch in 2019 is about 15,000 tons, and the transaction volume is about 450 million yuan. In 2017, the output of freshwater bass in Hunan Province was 3,600 tons, accounting for 0.79%of the country’s total output. There were more than 10 aquatic products distribution companies. Calculated by the proportion of circulation accounting for 90%of the total output, the trading volume of freshwater bass in Hunan in 2017 was about 3,200 tons. , The transaction volume is about 95 million yuan. In 2018, the output of freshwater bass in Hunan Province was 3,700 tons, accounting for 0.87%of the country’s total output. The transaction volume was about 3,300 tons, and the transaction volume was about 100 million yuan. It is estimated that the trading volume of Hunan freshwater perch in 2019 is 5,000 tons, and the trading volume is about 150 million yuan.
(6) Market consumption
1, market and price changes
(1) Price change trend
From 2017 to 2019, the average annual price of freshwater perch in China was 30.52, 27.23 and 29.49 yuan/kg, respectively. Tangkou price changes throughout the year have obvious seasonal changes. In the first four months of each year, the price remained relatively stable. From May to September, the price gradually rose to the highest price in the whole year. After October, the price began to fall until the beginning of the year due to the concentrated harvest at the end of the year. The changes in the monthly pond price of freshwater perch in my country from 2017 to 2019 are shown in Figure 2.
2, consumption intention and structure analysis
(1) Analysis of consumer preference for bass in different regions
bass acceptance. In the survey sample, there are fewer consumers who have never heard of bass, accounting for 3.84%of the total; who have heard of it but have not purchased 25.49%of the total; consumers who have purchased 70.67%. Among consumers who have never heard of bass, consumers in Tibet, Xinjiang, and Qinghai account for the highest proportions of the total local sample, respectively 19.23%, 9.38%, and 9.09%; among consumers who have heard of it but have not purchased Consumers in Xinjiang, Tibet, and Yunnan accounted for the highest proportions of the total local sample, with 53.13%, 50.00%, and 44.76%respectively. Among consumers who have heard of and purchased, consumers in Shanghai, Beijing, and Fujian accounted for The proportion of the total local sample is the highest, 87.18%, 81.73%, and 79.81%respectively. Most consumers have bought bass products. Consumers in the eastern region have a higher acceptance of bass (such as Shanghai, Beijing, Fujian), and consumers in the western region have the least acceptance of bass (such as Tibet, Xinjiang, and Qinghai).
Product purchase form. Among the main forms of products purchased, fresh and live products accounted for 77.74%, chilled products accounted for 12.81%, frozen products accounted for 6.67%, semi-finished products accounted for 1.64%, and ready-to-eat products accounted for 1.14%. It can be seen that the main consumption form of perch products is still Mainly live and fresh products. Provinces (cities, districts) where the proportion of consumers with fresh and live products as the main form of consumption exceeds 60%, including Shanghai, Jiangxi, Zhejiang, Henan, Jiangsu, Guangdong, Fujian, Shaanxi, Shanxi, etc.; fresh perch is second only to The second largest form of consumption of fresh perch, the proportion of consumers in various regions with ice fresh perch as the main form of consumption is 2%-20%, of which more than 10%of the provinces (cities, districts) include Tianjin, Liaoning, Shandong, Hebei , Beijing, Fujian, Jilin, Heilongjiang, Qinghai, Ningxia, Inner Mongolia, Shanxi and other places.
product purchase volume. Perch is not the main species of aquatic products consumed by consumers, and its consumption accounts for less than 30%of the total purchases of aquatic products. Consumers in the east account for 10-20%of total aquatic product consumption, 10-30%in the middle, and 5-20%in the west.
product price evaluation. The consumers who think that the price of bass are average accounted for 57.73%, the higher accounted for 31.31%, the lower accounted for 8.18%, the very high accounted for 2.11%, and the very low accounted for 0.67%. Consumers in the eastern, central and western regions have a more consistent evaluation of the price of perch, thinking it is fair or high.
(2) Analysis of different consumer groups’ consumption preferences for bass
Product purchase form. Fresh perch is the most common form of perch consumption. Young people (20 years old and below) have the lowest demand for fresh bass, and their consumption patterns are more diversified. Consumers aged 31-40 most prefer fresh bass, which is related to their existing fixed consumption habits. Consumers aged 20 and below who use live bass as the main form of consumption account for 43.96%of consumers in this age group, 21-30 years old for 54.77%, 31-40 years old for 60.73%, 41-50 years old for 58.73%, 51 Years old and above accounted for 48.80%. High-income consumer groups prefer fresh and live aquatic products. Consumers with a household monthly income of 5,000 yuan or less, whose main form of consumption is fresh perch, accounted for 43.35%of consumers of this income level, 5001-10000 yuan for 53.82%, 10001-15000 yuan for 57.55%, and 15001-20000 yuan for consumers. 63.71%, 2,0001-30000 yuan accounted for 67.01%, and more than 3,0001 yuan accounted for 63.74%.
product purchase volume. There is little difference in the amount of bass purchased by consumers of different age groups. Consumers aged 20 and under purchase bass in the proportion of total aquatic products mainly at 10-20%and below 5%. Consumers aged 21-30 are 5%-20%, 31-40 years old. The number of consumers is 5%-20%, the number of consumers aged 41-50 is 5%-20%, and the number of consumers aged 51 and above is 5%-20%. The lower the income level of consumers, the lower the percentage of perch purchases in the total purchases of aquatic products. Consumers with a monthly income of 5,000 yuan or less purchase perch in the proportion of total aquatic products mainly ≤10%, and consumers with a monthly income of 5,000 yuan or more purchase perch in the proportion of total aquatic products mainly concentrated in 520%.
product price evaluation. Older consumers are less able to pay and are more sensitive to the price of aquatic products. Most consumers aged 20 years and below, 21-30 years old, 31-40 years old, 41-50 years old think that the price of bass is average (38.05%, 41.69%, 41.38%, 40.57%), and most of them are 51 years old Consumers and above believe that the price of bass is higher (36.80%) and 5%-10%(19.20%). The level of income largely affects consumers’ perception of the price of high-quality goods. Consumers of different income levels have average or higher evaluations of the price of perch. The lower the income level, the higher the evaluation of the price.
Second, technology research and development progress
(1) Annual technical progress
1. Cultivation and promotion of fine varieties of largemouth bass
The Pearl River Fisheries Research Institute of the Chinese Academy of Fishery Sciences acquired the first new largemouth bass variety”Youlu No. 1″ in 2011 after five generations of selection, and its growth With fast speed and low deformity rate, breeding is promoted throughout the country, and the promotion area accounts for 50%of the national largemouth bass farming. In response to the slow growth of largemouth bass in the application of compound feed and the low efficiency of fry conversion and domestication, the largemouth bass”Youperch No. 3″, which is easy to eat compound feed and grows fast, was cultivated through group selection, 2019 Passed the approval of new varieties of aquatic products in 2015, it is expected to further promote the application of compound feed in largemouth bass breeding in my country through the promotion of new varieties, reduce the use of chilled fish, save fishery resources, and protect the aquaculture water environment.
In addition, in recent years, the characteristic freshwater fish industry system has carried out the selection and breeding of fine varieties of largemouth bass. Freshwater bass germplasm resources and variety improvement posts, engineered breeding posts, Hefei Comprehensive Experimental Station and Anhui enterprises jointly carried out selection and breeding research, using the original species of largemouth bass introduced from the United States in 2016 to carry out largemouth bass”Wanlu No. 1″”Cultivation. The genetic diversity of the introduced population is significantly higher than that of the existing domestic breeding populations. At present, three consecutive generations of breeding have been completed, and the growth speed and disease resistance performance are significantly better than the local breeding populations; in Guangdong, by selecting from multiple breeding populations The basic group, the largemouth bass”Jadefeng” strain, which has been selected and bred for three consecutive generations, also shows obvious growth advantages and high individual uniformity, which is very popular among farmers. The cultivation of fine varieties of largemouth bass can provide more high-quality seed for the rapidly expanding adult largemouth bass breeding industry in my country.
2. Research and development progress of efficient compound feed formulation technology
In recent years, relevant scientific research workers, especially the freshwater bass nutrition and feed team of the national characteristic freshwater fish industry system, have continuously improved the largemouth bass nutrition demand database. The scientific and precise nutrition regulation of largemouth bass has basically been realized. Good progress has been made in the main nutrient requirements, feed formulation improvement and processing technology.
In terms of sugar utilization, due to the low effective utilization of digestible sugar in feed for largemouth bass, excessive intake of digestible sugar can cause a series of harms, so Strictly controlling the intake of digestible sugars is the prerequisite for the development of high-efficiency feeds for largemouth bass. The study found that the appropriate digestible carbohydrate level in the feed for largemouth bass should be less than 10%, which is much lower than the recommended value of international counterparts (less than 19%). Too much sugar in the feed will cause excessive accumulation of liver glycogen, which in turn leads to the deviation of hepatocyte nuclei, vacuolation, and liver damage. Elevated blood sugar levels can stimulate insulin secretion and gene expression to a certain extent, but the activation of the insulin pathway cannot effectively inhibit the gluconeogenesis pathway, which is an important factor that causes its low glucose tolerance. At the same time, it studied the requirements of other important nutrients such as protein, fat, methionine, arginine, vitamin E and vitamin A in the feed for largemouth bass, providing a more comprehensive theoretical basis for formulating high-performance feed for largemouth bass . At the same time, based on the nutritional requirements and metabolic characteristics of largemouth bass, through the screening or improvement of the protein source, fat source, starch source and functional additives of the feed, a series of economical and practical feed formulas have been developed; The processing technology and key equipment that are compatible with the formula and feeding characteristics solve the key technology of low-starch feed processing.
The above-mentioned technological progress has played a great leading and promoting role in improving the industrial progress of my country’s high-efficiency special compound feed for largemouth bass. It is particularly worth mentioning that some special compound feed products for largemouth bass have been produced and used to prove very effective (feed coefficient is less than 0.9), can completely replace chilled fish feed, and have the advantages of fewer fish diseases. This will promote the transformation of my country’s largemouth bass farming model and help promote the green, efficient and sustainable development of the industry.
(2) Main recommended mode
1,”two mornings, one low and one high” breeding mode
In response to the problems of one-sided pursuit of output, excessively concentrated products on the market and low fish prices in the Pearl River Delta region’s aquaculture production, the national characteristic freshwater fish industry technology system has passed on-site Investigation and put forward the innovative breeding model of”two early, one low and one high” (ie:early release of seedlings, morning market, low density and high efficiency). Early release of seedlings:In the previous December or January of the year, the water flowers were stocked to cultivate fry in a greenhouse. At the end of February or early March, the fry were pulled out and counted, and then passed through the pond to the outer pond for adult fish breeding. Morning market:Set the time for the finished fish to go on the market from August to September. At this time, the finished fish will not be available, and the sales price is at a high level of the year, which is about twice as high as the price at the low level. Low density:Stocking size seedlings (10-15 g/tail) 4500-5000/mu, which is only 50%of the usual stocking density in the area; if the density is too high, the growth rate will slow down, which will affect the expectation of the morning market. High efficiency:After 3 years of this model, more than 6,000 mu of production practice has proved that the average net profit rate can reach 30,000 yuan/mu. The prerequisite (supporting facilities) for implementing this model is to build a plastic insulation shed for early fish breeding. The area of the insulation shed is about one-eighth of the adult fish breeding area.
2, off-season breeding and”996″ breeding mode
The production of largemouth bass seedlings in northern regions such as Jiangsu and Zhejiang is limited by the climate. The local seedling cultivation generally starts in April of that year, much later than the Pearl River Delta Regions, therefore, the market rate of finished fishes invested in seedlings that year was low, generally only about 40%, and the price of fish was also low. The remaining pond fish must go through the winter, and continue to breed until June to July in the next year before they can fully reach the finished fish specifications. These so-called”laokou fish” experienced overwintering and the following spring reproduction, and the loss was as high as 40%. Therefore, the low production rate of new fish that year, low fish prices, and the high loss of old fish in the following year are a major disadvantage of the largemouth bass industry in Jiangsu, Zhejiang and the northern regions. In this context, the national characteristic freshwater fish industry system combined with largemouth bass seedling companies after three years of exploration, through the regulation of environmental factors and key nutrients, has achieved a technological breakthrough in the production of largemouth bass in autumn reproduction. The region promotes the application of the”996″ breeding model (ie, the seedlings are released in September, after 9 months of breeding, and the finished fish will be marketed in June of the following year) to solve the bottleneck of seedlings. The advantages of this model are reflected in:(1) the loss of fish species overwintering is relatively small, no more than 20%; (2) the market is staggered, and the price of commercial fish is higher; (3) the benefit is higher than that of spring release. The production method of seedlings is on average about 50%higher. The prerequisite for implementing this model is the supply of autumn seedlings, and the price of autumn seedlings is about 50%higher than that of spring seedlings. However, this model is being favored by aquaculture producers and is expected to be widely promoted in Jiangsu, Zhejiang and other northern regions.
3, largemouth bass and river crab nest breeding technology
This aquaculture mode reduces the stocking density of a single species, and uses the different habitat and feeding characteristics of crabs and bass to build a”material-energy” ecological cycle and reduce environmental stress. , Thereby improving the quality, output value and economic benefits of aquaculture products while reducing the risk of breeding.
Technical focus:Make a reasonable match according to pond depth, aeration conditions, water source conditions, and aquatic conditions. The matching principle is:if the density of river crab is higher, the stocking density of largemouth bass should be appropriately reduced, and vice versa. The stocking density is 400-600 crabs/mu and 800-1200 largemouth bass/mu. Focus on the following tasks:(1) The off-season (early propagation) breeding of largemouth bass, to ensure that the fish fry can reach the stocking specifications when the water temperature is suitable for stocking from March to April; (2) The stocking is based on the feeding point. The center’s temporary nets are enclosed and fed at fixed points and regularly to allow the fry to form a fixed feeding habit (continue to tamed in the big pond); (3) The aquatic weeds in the early stage shall be managed according to the monoculture of crabs, and only bitter grass (noodles) shall be planted in the middle of the pond in the later stage. Grass), keep the water on the surface of the water free to circulate, cover 30-50%of the area of aquatic plants, and arrange the waterway and head to prevent it from growing out of the water. Properly put snails in the early stage of the breeding process to promote the conversion of feces and the ability to purify water quality. Provide natural bait. In recent years, the polyculture model of crab and bass has been demonstrated and promoted in Gaochun, Yixing, Siyang and other places in Jiangsu, covering an area of 4,000 mu, with an average benefit of about 5,000 yuan per acre, and good breeding benefits have been obtained.
(3) Development Trend Analysis
1. There is a huge demand for high-quality seed
my country’s largemouth bass consumer market has great potential, and because of the breakthrough and application of compound feed, the breeding area and scale of largemouth bass in my country have continued in the past three years. With the expansion, the demand for high-quality seedlings in various places is also increasing. Several main breeding areas have begun to cultivate seedlings locally to provide local seedlings. Because pond seedlings are greatly affected by climatic conditions and food organisms, factory seedlings and off-season seedlings have attracted a lot of seed producers to try. The technology of factory seedlings and off-season seedlings has made breakthroughs, which has promoted the scale of seedlings. produce.另一方面，种质资源与品种改良受到重视，多地开展大口黑鲈优良品种的培育，通过引进原种、台湾产的苗种以及对当地养殖群体的选育可促进我国大口黑鲈养殖业的健康持续发展。
美南伊利诺伊斯大学Adam等（2017）收集大口黑鲈野生群体相关数据建立贝叶斯多层模型，可预测最快上市的群体。快长骨骼肌中IGF I和IGF II的表达低于慢长群体，且后者的表达与体重显著负相关；Zhao等利用GBS测序挖掘大口黑鲈南方种全基因组SNP位点，其中58个SNPs 可100%鉴别亲本与子代（Zhao et al.，2018）。 Li等（2017）分析快长和慢长大口黑鲈肌肉转录组并筛选生长相关基因，鉴定出3个与生长相关的SNP标记；Bai等（2019）综述了大口黑鲈遗传育种和分子标记辅助选择育种技术进展。美学者对波多黎12个水库的遗传分析发现大口黑鲈南方种等位基因分布频率与十年前的估算值一致，南方种基因型占比0.03~0.64，且无北方种基因型（Peterson et al., 2017）。孙成飞等（2019）采用12对微卫星特异性荧光标记引物进行群体间遗传距离分析，显示现阶段中国大口黑鲈养殖群体的遗传多样性已显著下降。美康涅狄格大学Hessenauer等（2017）探讨渔业遗传管理方法，引入从未开发的大口黑鲈群体中的个体，以减轻休闲垂钓带来的种质选择压力。大口黑鲈超微观察发现其为硬骨鱼类Ⅰ型精子，结构与鲈形目其它种有异（乔志刚， 2017）；赵小兵等（2017）开展大口黑鲈早繁研究，可与广东完全同步批量供应苗种；Yan等（2019）证实大口黑鲈Dmrt1基因在性别决定和精巢发育中发挥着重要作用。
进一步完善了大口黑鲈营养需求数据库，通过摄食生长实验确定了大口黑鲈对蛋白质（Huang et al., 2017；Cai et al., 2019）、脂肪（Huang et al., 2017；Guo et al., 2019）、维生素A（连雪原等，2017）及维生素E（Li et al., 2018）的需求量。确定了饲料中适宜的淀粉源及添加水平（刘子科等，2017），发现淀粉水平的升高显著降低了大口黑鲈的生长性能（Ma et al., 2019），而淀粉水平过高则会导致氧化应激并抑制机体免疫力（Lin et al., 2018）。同时确定了适宜的淀粉种类（Song et al., 2018），豌豆淀粉可以在转录水平有效地提高大口黑鲈胰岛素通路的活力，进而减缓其肝糖原累积（Li et al., 2019a）。饲料中适宜水平的纤维素有助于促进生长性能及肝脏功能的维持（Zhong et al., 2019）。通过高通量测序，剖析了大口黑鲈对糖不耐受的分子机制（Zhang et al., 2019）。适宜的脂肪水平有助于提高生长性能（Guo et al., 2019），饲料脂肪升高会诱导大口黑鲈CPT1基因的表达（宋铭琪等，2019）。饲料中鱼油的氧化会严重损伤大口黑鲈的抗氧化系统，造成氧化应激，进而影响生长性能（Yin et al., 2019）。亚麻籽油的添加可有效降低大口黑鲈的脂肪沉积，显著提高其抗氧化能力（Shi et al., 2019）。在鱼粉替代方面，王孟乐等（2018）发现去皮豆粕分别与玉米蛋白和大米蛋白组成的植物性蛋白质混合物可使大口黑鲈饲料中的鱼粉含量由45%降至30%。普通豆粕、酶解豆粕与发酵豆粕均可替代30.91%的鱼粉（对照组鱼粉水平55%）， 且酶解豆粕的替代效果最好（张改改等，2019）。禽肉粉与豆粕的合理配伍可显著提高大口黑鲈摄食率以及饲料效率，替代饲料中60%的鱼粉（对组照鱼粉用量40%）（Ren et al., 2018）。在不影响生长及非特异性免疫等指标的前提下，鸡血浆粉可替代饲料中（鱼粉水平51%）10%的鱼粉（Li et al., 2019b）。饲料中的50%的鱼油可在不影响自身生长健康的情况下被豆油所替代，肉碱的添加有助于维持鱼油替代后机体的健康（Chen et al., 2020）。黄芪多糖及壳聚糖的适量添加显著提高大口黑鲈呼吸爆发活力，溶菌酶活性以及SOD活力从而增强鱼体免疫力及抗病力（Lin et al., 2017）。添加酵母水解物（Zhou et al., 2018; Gong et al., 2019）及抗菌肽（Li et al., 2020）有助于大口黑鲈非特异性免疫以及抗氧化性能的提升。 2.6-二叔丁基对甲酚（BHT）（Yu et al., 2018）、益生菌Grobiotic?-A（Yu et al., 2019）及肉碱（Chen et al., 2019）的适量添加可有效改善大口黑鲈的脂肪代谢以及抗氧化能力。
Ho等（2018）用诺卡氏菌的不同重组蛋白制备亚单位疫苗，发现其具有不同的保护效果。 Chen等根据诺卡氏菌的核糖体蛋白RpsA和RplL、FHA蛋白以及分子伴侣DnaK和GroEL制备DNA疫苗，发现其免疫保护率分别为78.31%、71.08%、79.33%、53.01%和80.71%，对诺卡氏菌病的预防有一定的应用前景（2019；2020）。 Zhang等（2019）用大口黑鲈弹状病毒的无毒株免疫大口黑鲈后用强毒株进行攻毒，发现其相对保护率达到100%，意味着该弱毒株在研发大口黑鲈弹状病毒疫苗上具有广阔的应用前景。袁等（2020）制备了大口黑鲈弹状病毒特异性卵黄抗体，该抗体对大口黑鲈弹状病毒具有明显的中和效果，为后续卵黄抗体作为免疫制剂的应用奠定了基础。