Wheat head blight caused by Fusarium graminearum is a major disease on wheat. In addition to causing serious yield and economic losses, pathogens can also produce mycotoxins to contaminate grain, posing a serious threat to human and animal health. At present, the control of wheat head blight mainly relies on chemical control. Due to long-term use of large quantities of drugs, pathogens have developed resistance to commonly used fungicides such as carbendazim. In-depth study and understanding of the functions and regulatory mechanisms of pathogenic proteins related to pathogenic bacteria will help to find new fungicidal targets and have important significance for the prevention and control of wheat head blight. Recently, the wheat disease prevention and control research innovation team of the Dry Farming Disease Research Office of the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences has continuously published articles in the authoritative journals of plant pathology”Molecular Plant-Microbe Interactions” and”Frontiers in Microbiology”, revealing The functions and regulatory mechanisms of two zinc finger proteins related to the pathogenicity of Fusarium graminearum are discussed.
A research paper entitled”The CHY-type zinc finger protein FgChy1 regulates polarized growth, pathogenicity, and microtubule assembly in Fusarium graminearum” published online on”Molecular Plant-Microbe Interactions” on December 28, 2020, report The important functions of CHY-type zinc finger protein FgChy1 in the growth, development and pathogenic process of Fusarium graminearum, as well as its molecular mechanism of regulating Beta tubulin assembly and mycelial polar growth.
This study found that the CHY-type zinc finger protein FgChy1 regulates the assembly of microtubules, and knockout mutants are more sensitive to targeted microtubule agents. Studies have found that FgChy1 controls the direction of mycelial growth (polar growth) by regulating the assembly of microtubules. This polarity regulation plays an important role in the formation and invasion of pathogen infection structures. Further studies have shown that FgCHY1 affects the positioning of the polar body complex protein FgSpa2-GFP at the top of the hypha by regulating the assembly of microtubules, thereby guiding the growth direction of the hypha. This study clarified the molecular function of the novel zinc finger protein FgChy1 and revealed its regulatory mechanism as a microtubule regulatory protein in the process of polar growth and pathogenicity of Fusarium graminearum.
”Functional characterization of calcineurin-responsive transcription factors Fg01341″ published on”Frontiers in Microbiology” on November 26, 2020 and Fg01350 in Fusarium graminearum”, reported the possibility of transcription factor Fg01341 as a Calcineurin-responsive zinc finger (Crz1) homologue in Fusarium graminearum, and proposed that Fg01350 can be used as a Crz1-independent homolog. The response factor of the pathway is involved in signal transduction. The regulatory network of the calcineurin signal pathway of Fusarium graminearum and the functions of related transcription factors are initially revealed.
The study found that the transcription factors Fg01341 and Fg01350 in Fusarium graminearum interact with the calcineurin catalytic subunit (CNA), respectively. The double knockout mutant is more present than the single gene deletion mutant. Severe phenotypic defect, but unlike Fg01341 which is regulated by calcineurin, which can transport from the cytoplasm to the nucleus, the location of Fg01350 does not depend on the catalysis of calcineurin. Studies have shown that the downstream transcriptional regulation pathways of calcineurin have two types:Crz1-dependent and independent, and the two pathway transcription factors have a synergistic effect in regulating the growth and development of Fusarium graminearum and the process of infection and disease.
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences is the first to complete the paper, researcher Chen Huaigu is the corresponding author, assistant researcher Dr. Shulin Cao and assistant researcher Zhang Xiangxiang (now working at the Jiangsu Coastal Research Institute) are two papers respectively The first author of the paper. The research was funded by the National Key R&D Program, Jiangsu Provincial Key R&D Program and the National Modern Agricultural Industrial Technology System Special Fund.
Link to the original text of MPMI:https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-20-0206-R
Front Microbiol original link:https://www.frontiersin.org/articles/10.3389/fmicb.2020.597998/full