On December 28, 2020, the team of Professor Hou Xilin of Nanjing Agricultural University made important progress in the genomics research of non-heading Chinese cabbage. The related results were named”A chromosome-level The reference genome of non-heading Chinese cabbage [Brassica campestris (syn. Brassica rapa) ssp. chinensis]” was published on Horticulture Research. After more than 10 years of in-depth research, the results revealed the metabolic mechanism of vitamin C and glucosinolate nutrients in non-heading cabbage, and laid a solid foundation for molecular design and breeding of excellent traits. At the same time, it also provides high-level reference genome data of non-heading cabbage for domestic and foreign counterparts.
Non-heading Chinese cabbage, commonly known as green cabbage and pakchoi, is originally from China and is one of the most popular vegetables. The cultivation area has increased from 8 million mu in 2005 It has risen to about 21 million mu and has become the third largest vegetable crop in my country. The research used the non-heading Chinese cabbage”Suzhou Green” as the material, using the latest sequencing methods to obtain a nearly complete and high-quality chromosome-level genome of 396.83Mb, and annotate its functions. Its ContigN50 2.83Mb. The quality of this genome is higher than other existing cabbage genomes. According to this new genome, the study found that repetitive sequences accounted for about 53%of the entire genome, and 48,158 protein-coding genes were annotated. Comparative genome analysis confirmed that non-heading Chinese cabbage experienced a genome-wide triplet (WGT) event shared with other cruciferous species, which occurred after the WGD event shared with Arabidopsis, for the purpose of studying Brassica Brassica The differentiation between species and subspecies provides an important basis. The genes related to the synthesis and metabolism pathways of vitamin C and glucosinolates (GSLs) were identified and comparatively analyzed, and the gene copy number variation caused by tandem replication was obtained, and the GSL gene was highly expanded in the cabbage genome. Combined with transcriptome analysis, the expression of related genes was explored, revealing the metabolic regulation mechanism of vitamin C and glucosinolate nutrients in non-heading Chinese cabbage.
This research work is based on the State Key Laboratory of Crop Genetics and Germplasm Innovation/Key Laboratory of Horticultural Crop Biology and Germplasm Creation in East China, Ministry of Agriculture and Rural Affairs/Horticulture The Engineering Research Center of the Ministry of Education for the Quality Innovation and Utilization of Biomass is the first unit, and Biomark Biotechnology Co., Ltd. is the second unit. Professor Hou Xilin from the College of Horticulture, Nanjing Agricultural University is the corresponding author of the paper. Professor Li Ying and Liu Gaofeng are the first authors of the same contribution. Associate Professors Liu Tongkun and Zhang Changwei from Nanjing Agricultural University, Lecturers Xiao Dong, Chen Fei and Biomark Biotechnology Co., Ltd. Ma Liming and Zheng Hongkun participated in the research. This research was approved by the National Key Research and Development Program (2016YFD0101701), the National Bulk Vegetable Industry Technology System (CARS-23-A-06), the National Natural Science Foundation of China (31872106), and the National Qingseng Vegetable Variety Major Scientific Research Joint Research Project (111821301354052283) Funding.