Recently, the animal genetic engineering and germplasm innovation team of the Institute of Animal Husbandry and Animal Husbandry Medical Institute has discovered key candidate genes related to the first division time of pig somatic cell cloned embryos. This discovery is an improvement Porcine somatic cell cloning embryo development efficiency provides new ideas and new methods. Related research results were published in”Genes”.
According to researcher Mu Yulian, pig somatic cell cloning technology is currently the only biotechnology that can obtain complete animal individuals through somatic cell genetic material, and is widely used in the creation of new breeding materials , Medical model construction, animal bioreactor construction, pig gene function research, high-quality breeding stock and endangered germplasm preservation, etc., have important scientific research value and application value. However, this technology still has problems such as low cloning embryo development ability and low cloning technology efficiency. The researchers used pig somatic cell cloned embryos as the research object. Through RNA library construction and sequencing, a total of 18460 genes were obtained, accounting for 71%of the annotated genes in the pig genome. By analyzing the difference between the early division group (first division time within 24 hours) and late division group (first division time within 36 hours), 3077 differentially expressed genes were obtained. Through GO and KEGG bioinformatics analysis, it is found that differentially expressed genes are significantly enriched in transcription and translation related pathways, especially ribosomal and rough endoplasmic reticulum protein processing pathways. Further analysis found that compared with late-division cloned embryos, the number of incorrectly expressed”donor cell memory genes” in early-division cloned embryos was less, suggesting that early-division cloned embryos have better reprogramming effects than late-division cloned embryos. The study found candidate genes related to the development of pig cloned embryos, which provided a theoretical basis for improving the development efficiency of pig cloned embryos and analyzing the mechanism of somatic cell cloning.
The research was supported by the National Natural Science Foundation of China and the basic scientific research business expenses of the Chinese Academy of Agricultural Sciences.
link to the original text:https://www.mdpi.com/2073-4425/11/12/1499#